技術原理
單克隆抗體是由單一B細胞克隆產生的高度均一、僅針對某一特定抗原表位的抗體。它在藥物研發、診斷試劑開發中扮演了關鍵的角色。抗體分子本的一級結構，特別是它的CDR區域氨基酸序列是其發揮生物學功能的核心。因此，對單抗分子進行準確快速的鑒定分析具有重要的意義。通過先進的高分辨率質譜儀Obitrap Fusion Lumos，結合豐富的生物信息學分析經驗，可以對不同亞型抗體（例如IgG和IgM）和不同類型抗體（熒光偶聯抗體，固定化抗體，不同物種的抗體）的一級序列信息進行快速準確的分析。
 
 

單克隆抗體測序技術原理圖

技術特點
l  不基于核酸測序與雜交瘤細胞；
l  獲得抗體的輕重鏈完整氨基酸，可以直接表達；
l  能夠區分I/L;
l  周期快（20天，加急2周出結果）
 
樣品要求
l  純度>95%(SDS-PAGE膠圖，HPLC或者是質譜分子量證據，可提供純度更低的樣品解析服務，具體電聯）；
l  只需要少至100ug樣品（可提供更低樣品量的解析服務，具體電聯）；
 
費用返還保證

注意事項
l  請使用足量的干冰運輸，并盡量選用較快的郵遞方式，以降低運輸過程中樣品降解的可能性。
 
項目報告
l  實驗步驟
l  質譜參數
l  質譜譜圖
l  單抗分子序列的詳細信息
 
單克隆抗體測序一站式服務
l  您只需下單-寄送樣品
l  明德正康為您完成-樣品處理-上機分析-數據分析-項目報告

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